Rapid and sensitive diagnosis of plant quarantine fungi <i>Alternariatriticina</i> and <i>Plenodomuslibanotidis</i> based on the RPA-CRISPR/Cas12a system.

ABSTRACT: With the increase in cross-border transmission in the context of globalization, the necessity for developing rapid and accurate detection methods for plant pathogens has become critical. This study introduces a recombinase polymerase amplification (RPA) technique combined with CRISPR/Cas12a cleavage and fluorescence-based detection systems (FRB) or paper-based lateral flow strips (PLFS) for the rapid on-site detection of invasive alien fungi, specifically Alternariatriticina and Plenodomuslibanotidis, which pose significant threats to agriculture and biodiversity. The results demonstrate that either RPA-CRISPR/Cas12a-FRB or RPA-CRISPR/Cas12a-PLFS can accurately detect the target species within 30 min, with a sensitivity of up to 10 pg/μL. These portable and easy-to-use assays

SUBMITTER: Phurbu D 

PROVIDER: S-EPMC12177518 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature