An N-Terminally Elongated Peptide From Conus rolani Defines a New Class of Ribbon α-Conotoxins Targeting Muscle nAChRs.

ABSTRACT: Conotoxins, peptides found in cone snail venoms, selectively target ion channels and receptors to incapacitate prey. α-Conotoxins are extensively investigated for their potent modulation of nicotinic acetylcholine receptors (nAChRs). This study describes the discovery and characterization of RoIA, a novel α-conotoxin from Conus rolani. RoIA belongs to the α4/4 conotoxin class and features an atypical N-terminal elongation of 18 amino acids. The biological activity of RoIA is assessed through both in vivo and in vitro assays using the three potential folding isoforms (globular, ribbon, and bead) of the full-length and truncated (lacking the N-terminal elongation) analogs. The full-length RoIA analog exhibits delayed but potent paralytic activity when administered to mice and fish; the ribbo

SUBMITTER: Giglio ML 

PROVIDER: S-EPMC12178101 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature