Low-Glucose Culture Conditions Bias Neuronal Energetics Towards Oxidative Phosphorylation.

ABSTRACT: Neurons are almost exclusively cultured in media containing glucose at much higher concentrations than found in the brain. To test whether these "standard" hyperglycemic culture conditions affect neuronal respiration relative to near-euglycemic conditions, we compared neuronal cultures grown with minimal glial contamination from the hippocampus and cortex of neonatal C57BL/6NCrl mice in standard commercially available media (25 mM Glucose) and in identical media with 5 mM glucose. Neuronal growth in both glucose concentrations proceeded until at least 14 days in vitro, with similar morphology and synaptogenesis. Neurons grown in high glucose were highly dependent on glycolysis as their primary source of ATP, measured using ATP luminescence and cellular respirometry assays. In contrast, neu

SUBMITTER: Swain S 

PROVIDER: S-EPMC12178110 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature