Multi-omics analysis of yolk and allantoic fluid in chicken embryonic development and sexual differentiation.

ABSTRACT: The laying hen's industry faces significant ethical and economic challenges due to day-old male chick culling, driving the need for reliable in ovo sexing methods. While chicken embryo allantoic fluid (AF) shows promise for early sex determination through sex-specific biomarkers, current research lacks comprehensive sex-specific analyses of fertilized egg AF. Moreover, AF production and metabolism involve dynamically complex, multi-component regulatory interactions that require further investigation. To address this knowledge gap, we systematically investigated the sex-specific molecular characterization of egg yolk and AF from day 1 and day 12 of chicken embryo development using metabolomics and proteomics techniques. Egg yolk proteomics analysis showed that the metabolic requirements of

SUBMITTER: Xu S 

PROVIDER: S-EPMC12178797 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature