The N-6 methyladenosine dynamics in STEMI and the effect of IL-6 inhibition - a hypothesis generating sub-study of the ASSAIL-MI trial.

ABSTRACT:

Background

Epitranscriptomics, with m⁶A as the most prevalent in mammals, is a novel treatment target for inflammatory diseases, including cardiovascular diseases. However, little is known about m⁶A RNA-regulation during myocardial infarction (MI).

Methods

In this explorative sub-study of the ASSAIL-MI trial, we used whole blood samples from patients with acute ST-elevation MI (STEMI) (n=6) at admission and after 3-7 days, and from healthy control subjects (n=3). RNA was isolated, and m⁶A sites were analyzed using human m⁶A single nucleotide resolution microarray analysis. mRNA levels were analyzed using RNA sequencing analysis.

Results

Compared with controls, patients with STEMI had a strikingly different pattern of m⁶

SUBMITTER: Dahl TB 

PROVIDER: S-EPMC12178860 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature