<i>Culicoides</i> allergens expressed in insect cells induce sulphidoleukotriene release from peripheral blood leukocytes of horses affected with insect bite hypersensitivity.

ABSTRACT:

Introduction

Insect bite hypersensitivity (IBH) is an IgE-mediated dermatitis in horses caused by bites of Culicoides spp. The allergens are salivary gland proteins from these insects, and nine major allergens from Culicoides obsoletus have been identified and expressed in E. coli. However, proteins expressed in procaryotic systems have limitations in cellular assays, particularly in functional assays assessing the allergen-induced release of mediators in vitro, such as sulphidoleukotrienes (sLT) from basophils. The aims of the study were to produce functional Culicoides allergens in insect cells, to assess their allergenicity using a sLT release assay, and to relate the sLT release with IgE sensitization to the respective allergens using ELISA.

SUBMITTER: Jonsdottir S 

PROVIDER: S-EPMC12178880 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature