The P-loop NTPase RUVBL2 is a conserved clock component across eukaryotes.

ABSTRACT: The eukaryotic circadian clock keeps time by using a transcription-translation feedback loop, which exhibits an architecture that is conserved across a diverse range of organisms, including fungi, plants and animals¹. Despite their mechanistic similarity, the molecular components of these clocks indicate a lack of common ancestry². Our study reveals that RUVBL2, which is a P-loop NTPase enzyme previously shown to affect circadian phase and amplitude as part of mammalian clock super-complexes, influences the circadian period through its remarkably slow ATPase activity, resembling the well-characterized KaiC-based clock in cyanobacteria. A screen of RUVBL2 variants identified arrhythmic, short-period and long-period mutants that altered circadian locomotor activity rhyt

SUBMITTER: Liao M 

PROVIDER: S-EPMC12178907 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature