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Establishing a co-culture aggregate of N-cycle bacteria to elucidate flocculation in biological wastewater treatment.


ABSTRACT: Biological flocculation is a complex phenomenon that is often treated as a black box. As a result, flocculation problems are usually remediated without knowledge of the exact causes. We show that it is feasible to exploit a model (N-cycle) consortium with reduced complexity to fundamentally study bioflocculation. Strong nitrifier microcolonies were formed during oxic/anoxic cycles in sequencing batch reactors, using alginate entrapment as a cell retention system. After the release of these aggregates into suspension, macroclusters with flocs of the denitrifier were observed. These results suggest that a living model of a full-scale activated sludge floc can be built through the use of this bottom-up approach. By eliminating shifts in the microbial community, the applied experimental condit

SUBMITTER: Parret L 

PROVIDER: S-EPMC12178987 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature