ABSTRACT: Target leaf spot disease (TLS), caused by Corynespora cassiicola (Berk & Curt) Wei, ranks among the most serious fungal diseases affecting cucumber production. However, the genetic basis for TLS resistance in cucumber has not yet been determined. In this study, we evaluated TLS resistance in the adult plants of 130 cucumber accessions using a disease index (DI) in October 2021, June 2023, and October 2023. The accessions used in this study were representative collection selected from the global 3,342 accessions, and contain four ecotypes (the Eurasian, Indian, East Asian, and Xishuangbanna type). Cluster analysis suggested that 11 of the 130 accessions exhibited high levels of TLS resistance (CG28, CG70, CG84, CG86, CG100, CG104, CG98, R163, R61, CG64, CG71). A genome-wide associati
