Single-cell and spatial transcriptomics reveal a stress-induced EMT-like epithelial subset driving immune activation in silica-injured lung.

ABSTRACT: The mechanism that lung epithelial cells regulate immune responses during chronic injury still remains unclear. Here, we combined single-cell RNA sequencing with spatial transcriptomics to track epithelial dynamics in silica (SiO₂)-exposed mouse lungs. By day 56, SiO₂ induced significant epithelial proliferation, followed with a distinct C0 subset emerging as the dominant population. C0 cells co-expressed surfactant genes (Sftpc, Scgb3a2), mesenchymal markers (Vim, Mmp12), and pro-inflammatory cytokines (Ccl6, S100a8/a9), reflecting a hybrid phenotype. Spatial and cell-cell interaction analyses showed C0 cells engaging macrophages and neutrophils through SPP1-CD44, APP-CD74, and GRN-MARCO signaling, driving immune recruitment

SUBMITTER: Wang T 

PROVIDER: S-EPMC12179088 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature