<i>Bradyrhizobium tunisiense</i> sp. nov., a novel rhizobial species isolated from <i>Acacia saligna</i> nodules.

ABSTRACT: Three slow-growing rhizobial strains, designated as 1AS2L^T, 1AS20L and 1AS5L, were isolated from nodules of Acacia saligna in Borj Cedria, northern Tunisia. These strains, which belong to the symbiovar cyanophyllae, were characterized using a polyphasic approach. Phylogenetic analysis of the 16S rRNA (rrs) gene placed these strains within the genus Bradyrhizobium, specifically in the superclade associated with Bradyrhizobium japonicum. Further phylogenetic analysis using concatenated sequences of the recA, atpD, glnII and gyrB genes (totalling 1,734 bp) positioned the strains in a distinct lineage, with Bradyrhizobium shewense identified as their closest related species, sharing a sequence identity of 95.2%. The type

SUBMITTER: Hsouna J 

PROVIDER: S-EPMC12179390 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature