Characterizing the Impact of Primer-Template Mismatches on Recombinase Polymerase Amplification.

ABSTRACT: Recombinase polymerase amplification (RPA) is an isothermal amplification assay that has been ubiquitously utilized in the detection of infectious agents. Like any nucleic acid amplification technology, primer-template complementarity is critical to RPA reaction success. Mismatches arising in the primer-template complex are known to impact reaction kinetics, invalidate downstream analysis, such as nucleic acid quantification, and result in false negatives if used in a diagnostic capacity. Although the impact of specific primer-template mismatches has been well characterized for techniques such as PCR, characterization remains limited for RPA. Through our study, we systematically characterize the impact of mismatches on the RPA reaction, when located in the 3'-anchor region of the primer-te

SUBMITTER: Higgins M 

PROVIDER: S-EPMC12179515 | biostudies-literature | 2022 Nov

REPOSITORIES: biostudies-literature