GSK3A promotes human adenovirus replication and phosphorylates viral L4-22K protein.

ABSTRACT: Human adenovirus B7 (HAdV-B7) is a significant respiratory pathogen in children, associated with substantial morbidity and mortality. Despite its clinical importance, the molecular mechanisms of HAdV-B7-host interaction remain poorly elucidated. In this study, we performed a high-throughput gain-of-function cDNA library screening and identified glycogen synthase kinase 3α (GSK3A) as a key proviral factor facilitating HAdV-B7 replication. The overexpression of GSK3A enhanced viral replication, whereas knockdown or knockout inhibited it. Furthermore, the kinase-active S21A mutant significantly augmented viral replication, whereas the kinase-inactive Y279A and K148A mutants of GSK3A failed to support it, highlighting the importance of its kinase activity on HAdV-B7 replication. Notably, phosp

SUBMITTER: Lin Y 

PROVIDER: S-EPMC12179656 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature