Comparative transcriptome analysis reveals candidate gene for flowering time QTL HvHeading in barley.

ABSTRACT:

Background

Identifying genes regulating flowering time enhances understanding mechanisms that improve crop adaptation and productivity. This study aims to identify gene(s) underlying barley flowering time quantitative trait locus (QTL) "HvHeading".

Results

To investigate the reported delayed-flowering effect of QTL, we selected spring barley MAGIC DH lines with different alleles in HvHeading locus which carry the same alleles in epistatic loci. Phenotyping apex development revealed contrasting effects of two alleles of HvHeading locus. Combining recombination and differential gene expression analyses using RNA-sequencing for apex and leaf tissue pinpointed a 3.94 Mbs interval which carried 22 differently expressed genes. Initial analysis using Morex IBSC v2 reference genome

SUBMITTER: Afsharyan NP 

PROVIDER: S-EPMC12180222 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature