Cold-blooded vertebrates evolved regulatory B cells to participate in inflammatory diseases.

ABSTRACT:

Background

Regulatory B cell (Breg), known for its immunosuppressive properties through the provision of IL-10, plays a critical role in the control of inflammatory diseases. Although Breg has been discovered for over two decades in mammals, its existence in non-mammalian vertebrates remains unclear. Here, we aimed to explore the differentiation mechanism and functional profiles of teleost CD25L⁺ Breg to gain insights into the origin and evolution of Breg.

Methods

Flow cytometry, RNA-seq, qPCR, morphological analysis, immunoblotting, immunofluorescence, recombinant IL-35 stimulation, cell co-culture in Transwell system were performed to reveal the phenotypic features, differentiation mechanism and suppressive functions of teleost CD25L⁺ Breg. To elucida

SUBMITTER: Wang J 

PROVIDER: S-EPMC12180223 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature