Role of antibody heavy and light chain interface residues in affinity maturation of binding to HIV envelope glycoprotein.

ABSTRACT: The F_V region of an antibody consists of the heavy chain (HC) and light chain (LC) variable domains whose association is maintained by a series of conserved, non-polar interactions. During chronic infections, somatic mutations are induced, often in the HC/LC interface. Sequence variation in these interactions allows the HC and LC domains to inhabit a range of orientations relative to one another. Thus, we hypothesize that these interface mutations are critical to orient and rigidify the HC/LC interface to arrange the paratope for optimal interaction with the antigen, thereby affecting antigen binding affinity allosterically. To test this hypothesis, we measured the HC/LC orientation of a set of broad and potent human HIV neutralizing antibodies. The HC/LC interface of these anti

SUBMITTER: Cisneros A 

PROVIDER: S-EPMC12180580 | biostudies-literature | 2019 Aug

REPOSITORIES: biostudies-literature