VPA Enhances Pulp Regeneration by Regulating LPS-Induced Human Dental Pulp Stem Cells Odontogenic Differentiation.

ABSTRACT:

Aim

This study aimed to investigate the effect of valproic acid (VPA) exposure on pulp inflammation and regeneration.

Methods

The effects of VPA on pulp regeneration and reparative dentin formation were examined using cone-beam computed tomography (CBCT) and histological staining in vivo after 3 months of endodontic regenerative procedures (ERPs). In vitro, the effect of VPA on the odontogenic differentiation of human dental pulp stem cells (hDPSCs) was assessed using mineralization assays following lipopolysaccharide (LPS) exposure. Western blotting, quantitative real-time polymerase chain reaction, and reverse transcriptase polymerase chain reaction were performed to analyze changes in gene and transcript expression. Mineralization and transfection assays were conducted to

SUBMITTER: Wang Z 

PROVIDER: S-EPMC12180980 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature