Key amino acids in RNA polymerase and helicase proteins regulate RNA synthesis efficiency in porcine reproductive and respiratory syndrome virus.

ABSTRACT: Porcine reproductive and respiratory syndrome virus (PRRSV) exhibits rapid evolution due to its high mutation rate and frequent recombination, posing significant challenges for disease control. In this study, we investigated the molecular mechanisms underlying strain-specific variations in PRRSV replication phenotypes. Using reverse genetics and molecular biology approaches, we established a non-infectious replicon model that simulates PRRSV genomic replication and subgenomic (sg) mRNA transcription at the cellular level. This model enabled the evaluation of regulatory effects of viral non-structural proteins (nsps) and transcription-regulating sequences (TRSs) on viral replication and transcription, revealing the crucial roles of nsp9 and nsp12 in RNA synthesis. Furthermore, we developed

SUBMITTER: Li H 

PROVIDER: S-EPMC12180982 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature