A label-free electrochemical immunosensor on gold-printed electrode for the electrochemical detection of C-reactive protein from blood samples.

ABSTRACT: The fabrication of a simple, label-free, electrochemical immunosensor for C-reactive protein (CRP) detection is presented using a custom-designed printed circuit board (PCB) gold electrode. To enhance the sensitivity of the sensing platform, the surface of the gold working electrode was modified with multi-walled carbon nanotubes (MWCNTs), leveraging its exceptional conductivity and biocompatibility. The label-free immunosensor operates on immunological principles, with anti-CRP antibodies covalently immobilized on the modified electrode to capture and quantify CRP levels, using differential pulse voltammetry (DPV). The immunosensor demonstrated high specificity to CRP, exhibiting a linear detection range of 1.25 to 80 μg/mL of CRP in 1 × PBS, and achieving a detection limit (LOD) of 0.745

SUBMITTER: Zambry NS 

PROVIDER: S-EPMC12181133 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature