Baseline CBV-normalized BOLD fMRI of visual stimulation in awake vs. anesthetized mice.

ABSTRACT: Anesthesia profoundly affects baseline physiology, including CBV and evoked BOLD fMRI responses. To disentangle evoked BOLD responses into CBV and pure BOLD components, we conducted mouse fMRI experiments at 15.2 T using 5 Hz visual stimulation and a 4-second 10% O₂ hypoxic stimulus under awake and 0.5% isoflurane-anesthetized conditions. With a newly developed mouse cradle that allowed free movement of the paws, no obviously unusual behaviors were observed, and head motion was acceptable even without habituation. Anesthesia increased baseline CBV globally, impacting evoked BOLD responses. Visual stimulation elicited significant BOLD responses in visual networks, including the lateral geniculate nucleus, superior colliculus, lateral posterior thalamus, and visual cortical areas

SUBMITTER: Choi SH 

PROVIDER: S-EPMC12181191 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature