Age-related changes in the proteome and mitochondrial metabolism of rabbit adipose-derived stromal/stem cells.

ABSTRACT: Adipose tissue is continuously regenerated by stromal mesenchymal stem cells throughout life. This study hypothesises that early age-related changes in the proteome and metabolic properties of subcutaneous (s) and visceral (v) adipose tissue-derived stromal/stem cells (ASCs) from young and old rabbits contribute to a loss of stem cell plasticity and function. To test this, the proteome and metabolic properties of ASCs from young and old rabbits were analysed using mass spectrometry-based label-free quantification and mitochondrial respiration measurements (Seahorse Mito Cell Stress Test). Both sASCs and vASCs from old rabbits exhibited comparable clusters of differentially expressed proteins. However, age-related changes were more pronounced in sASCs, suggesting that ageing affects ASCs di

SUBMITTER: Toto Nienguesso A 

PROVIDER: S-EPMC12181353 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature