Distinct transcriptomic signatures in pulmonary tissue resident and vascular resident-like T cells in nonhuman primates.

ABSTRACT: The concept of intravascular immunity is reshaping our understanding of immune surveillance, challenging the traditional view that immune responses are confined to either tissue parenchyma or the bloodstream. Our pilot study utilized intravascular staining (ivs) in rhesus macaques (RhM) to spatially distinguish pulmonary vasculature-associated (ivs+) CD8⁺ T cells from interstitial (ivs-) T cells. Single-cell RNA sequencing and flow cytometry revealed that ivs+ T cells are not passive blood contaminants but rather a distinct, "resident-like" population enriched in cytotoxic effectors, with elevated expression of transcripts associated with tissue residency, cell adhesion, and vascular/platelet interactions, while ivs- T cells exhibited a classic T_RM signature. The pres

SUBMITTER: Melton A 

PROVIDER: S-EPMC12182324 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature