Imaging Ligand-Receptor Interactions at Single-Protein Resolution with DNA-PAINT.

ABSTRACT: Ligand-receptor interactions are critical for cell communication, with membrane receptors such as the Epidermal Growth Factor Receptor (EGFR) mediating responses to external signals. Super-resolution microscopy techniques in principle allow the visualization of these interactions at single-molecule resolution. While DNA-Points Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) super-resolution microscopy has been successfully used to image receptors, specific labeling of cognate ligands, such as EGF, with DNA has remained challenging. Here, an approach to label and image the small extracellular ligand EGF using site-specific tagging and DNA modification is presented. Functional, site-specifically tagged EGF constructs, including DNA-conjugated and ALFA-tagged EGF, are generated.

SUBMITTER: Honsa M 

PROVIDER: S-EPMC12182887 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature