RNA G‑Quadruplex Reprogramming with Guanine-Rich Antisense Oligonucleotides Inhibits Monoamine Oxidase B's Translation.

ABSTRACT: The human transcriptome contains secondary RNA structures like RNA G-quadruplexes (rG4s) which regulate biological processes such as translation by ribosome stalling. Canonical rG4s, which are stabilized by both Hoogsteen hydrogen bonds and potassium ions, are known to hinder translation in the 5' untranslated region (5'UTR) of mRNAs. In neurodegenerative diseases, including Parkinson's disease (PD), rG4s have been shown to influence protein synthesis. However, the impact of rG4s in nonmutated therapeutic targets like monoamine oxidase B (MAOB), an enzyme involved in dopamine metabolism, remains unexplored. In this study, an rG4 located in the MAOB mRNA's 5'UTR was identified, and ways to either stabilize or reprogram this rG4 were explored. The translation inhibitory role of the rG4 was d

SUBMITTER: Turcotte MA 

PROVIDER: S-EPMC12183519 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature