Mechanism of Forced-Copy-Choice RNA Recombination by Enteroviral RNA-Dependent RNA Polymerases.

ABSTRACT: Forced-copy-choice recombination occurs at the end of a template, differing from copy-choice recombination, which happens at internal positions. This mechanism may produce full-length genomes from fragments created by host antiviral responses. Previous studies from our laboratory demonstrated that poliovirus (PV) RNA-dependent RNA polymerase (RdRp) switches to an "acceptor" template in vitro when initiated on a heteropolymeric RNA-primed "donor" template. Surprisingly, recombinants showed template switching from the 3'-end of the donor template. We have developed a primed-template system to study PV RdRp-catalyzed forced-copy-choice RNA recombination. PV RdRp adds a single, nontemplated nucleotide to the 3'-end of a blunt-ended, double-stranded RNA product, forming a "plus-one" inte

SUBMITTER: Arnold JJ 

PROVIDER: S-EPMC12183593 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature