Co-opted SUMO machinery promotes condensate formation associated with membranous replication organelles of a positive-strand RNA virus.

ABSTRACT: Positive-strand RNA viruses are important pathogens of humans and plants. These viruses built viral replication organelles (VROs) with the help of co-opted host proteins and intracellular membranes to support robust virus replication in infected cells. Tomato bushy stunt virus (TBSV), a model (+)RNA virus, assembles membranous VROs, which are associated with vir-condensate substructures driven by TBSV p33 replication-associated protein. In this work, we provide evidence that the peroxisome-associated TBSV and the mitochondria-associated carnation Italian ringspot virus hijack the host small ubiquitin-like modifier (SUMO) machinery in yeast model host and plants. Based on knockdown of components of the SUMO pathway, we show that SUMO machinery acts as a cellular proviral dependency factor d

SUBMITTER: Lin W 

PROVIDER: S-EPMC12184518 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature