Impact of nephrotoxins and oxidants on survival and transport function of hiPSC-derived renal proximal tubular cells.

ABSTRACT: Due to their role in excretion, renal proximal tubular cells are susceptible to damage by toxic metabolites and xenobiotics. The regenerative capacity of the kidney allows for the replacement of damaged cells, a process involving differentiation programs. However, kidney function tends to decline, suggesting that the replacement cells may not achieve full functionality. To understand possible causes of this decline, we investigated effects of nephrotoxins and oxidants on the differentiation of induced pluripotent stem cells (iPSC) into proximal tubular epithelial-like cells (PTELC). Proliferation, apoptosis, senescence, and expression of oxidative defense genes were analyzed in iPSC, differentiating and differentiated cells treated with cisplatin (CisPt, up to 45 µM), cyclosporin A (CycA,

SUBMITTER: Mboni-Johnston IM 

PROVIDER: S-EPMC12185579 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature