Developing a new cleavable crosslinker reagent for in-cell crosslinking.

ABSTRACT: Crosslinking mass spectrometry (XL-MS) is a powerful technology that recently emerged as an essential complementary tool for elucidating protein structures and mapping interactions within a protein network. Crosslinkers which are amenable to post-linking backbone cleavage simplify peptide identification, aid in 3D structure determination and enable system-wide studies of protein-protein interactions (PPIs) in cellular environments. However, state-of-the-art cleavable linkers are fraught with practical limitations, including extensive evaluation of fragmentation energies and fragmentation behavior of the crosslinker backbone. We herein introduce DiSPASO (bis(2,5-dioxopyrrolidin-1-yl) 3,3'-((5-ethynyl-1,3-phenylene)bis(methylenesulfinyl))dipropanoate) as a lysine-selective, MS-cleavable cros

SUBMITTER: Muller F 

PROVIDER: S-EPMC12185727 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature