Transcriptomic profiling reveals RetS-mediated regulation of type VI secretion system and host cell responses in <i>Pseudomonas aeruginosa</i> infections.

ABSTRACT: Pseudomonas aeruginosa is a major opportunistic pathogen that causes chronic infections, particularly in patients with cystic fibrosis and chronic obstructive pulmonary disease (COPD). The type VI secretion system (T6SS) is a primary virulence factor of P. aeruginosa in chronic infections. The objective of this study was to elucidate the regulatory mechanisms and pathogenic effects of the T6SS during P. aeruginosa infection, utilizing transcriptome sequencing and functional assays. We found that T6SS expression is elevated in P. aeruginosa isolated from chronically infected patients. Deletion of the retS gene activates P. aeruginosa PAO1 T6SS while repressing T3SS in vitro. Bacterial and cellular transcriptome sequencing analyses showed that

SUBMITTER: Wu Y 

PROVIDER: S-EPMC12185982 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature