Functional characterization of transcriptional enhancers in an Anopheles genetic locus controlling natural resistance to the malaria parasite, Plasmodium falciparum.

ABSTRACT:

Background

Anopheles mosquitoes and the malaria parasites they transmit remain a significant global health problem. Most genomic and functional genomic studies of mosquitoes have focused on the protein-coding genome, and comparatively little is known about the importance of noncoding transcriptional enhancers in controlling their gene expression and phenotypic variation. Here we evaluate nine enhancers previously identified in a STARR-seq screen and present in a genetic locus that was identified as a major influence on susceptibility to malaria infection in wild Anopheles coluzzii mosquitoes.

Result

We developed an analytical pipeline to filter nine enhancers in the malaria susceptibility locus on chromosome 2L. First, ATAC-seq revealed that only three of the nine enhancers

SUBMITTER: Zmarlak-Feher NM 

PROVIDER: S-EPMC12186386 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature