Authentication of the Botanical Origin of Honey: <i>In Silico</i> Assessment of Primers for DNA Metabarcoding.

ABSTRACT: Honey is a valuable food product that is often mislabeled as to its botanical composition, affecting both trade transparency and consumer safety. DNA metabarcoding is a promising method for authenticity verification, although the results are affected by the choice of molecular markers and primer pairs. This study selected 43 primer pairs from the literature covering several genes (chloroplast rbcL, matK, and trnL and nuclear ITS) and length of <600 bp. Performance of these primers was in silico assessed for all Streptophyta and a subset of them representing the honeybee foraging plants included in the European list. The results showed that rbcL primers provided the highest taxonomic coverage, which increased significantly when combined with ITS2

SUBMITTER: Ranieri L 

PROVIDER: S-EPMC12186523 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature