Standardized Quorum Sensing Tools for Gram-Negative Bacteria.

ABSTRACT: Engineering synthetic consortia to perform distributed functions requires robust quorum sensing (QS) systems to facilitate communication between cells. However, the current QS toolbox lacks standardized implementations, which are particularly valuable for use in bacteria beyond the model species Escherichia coli. We developed a set of three QS systems encompassing both sender and receiver modules, constructed using backbones from the SEVA (Standard European Vector Architecture) plasmid collection. This increases versatility, allowing plasmid features like the origin of replication or antibiotic marker to be easily swapped. The systems were characterized using the synthetic biology chassis Pseudomonas putida. We first tested individual modules, then combined sender and receive

SUBMITTER: Mugica-Galan P 

PROVIDER: S-EPMC12186672 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature