Quantifying the influence of optical coherence tomography beam tilt in each retinal layer.

ABSTRACT: PurposeWell-aligned microstructures within the retina - like retinal nerve fiber layer (RNFL) axons - differentially reflect light depending on its angle. Our goal was to quantify the influence of optical coherence tomography (OCT) beam tilt on reflectivity of each layer of the mouse retina. MethodsWe collected OCT images in a single plane capturing the optic nerve head, temporal retina, and nasal retina, while tilting the OCT beam at various angles. We converted signal intensities to estimated attenuation coefficients (eAC). The attenuation coefficient describes how quickly the remainder of an OCT beam's light is absorbed or scattered at a given depth into the retina. A single-ellipse model based on prior literature was calculated at each retinal depth, describing the maximum eAC across a

SUBMITTER: Bissig D 

PROVIDER: S-EPMC12186825 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature