Protein landscape of the brush border membrane of first instar larvae of Frankliniella occidentalis, the western flower thrips.

ABSTRACT: We investigated the protein composition of the brush border membrane of larval Frankliniella occidentalis (western flower thrips), an agriculturally significant crop pest and vector of plant pathogens. We developed a protocol for purifying brush border membrane vesicles (BBMVs) from first-instar larvae (L1) bodies and identified their protein composition by LC-MS/MS. From 2544 proteins identified, 469 were predicted to be secreted and part of the inner and outer plasma membrane leaflet using cell-localization prediction tools and homology with reviewed proteins in the UniProt database. Comparison to thrips tissue-specific proteomes revealed that 371 and 263 of the identified BBMV plasma membrane and secreted proteins matched the larval gut and adult salivary glands, respectively. Annotatio

SUBMITTER: Maurastoni M 

PROVIDER: S-EPMC12186882 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature