Downregulation of Endo-Beta-N-Acetylglucosaminidase in Caenorhabditis elegans Improves Stress Adaptivity.

ABSTRACT:

Introduction

Endo-beta-N-acetylglucosaminidase (ENGASE) is one of the key enzymes involved in the structural and functional regulations of glycoproteins. Although its enzymatic activities and applications have been well studied in vitro, its biological function in vivo yet remains to be illustrated. In this study, the biological function of ENGASE in Caenorhabditis elegans was explored in detail.

Methods

An Engase gene knockout in C. elegans (CeEng-1 or CeEngase) was constructed and subjected to a panel of phenotypical and glycomics analysis. In addition, in vitro and in vivo ENGASE inhibition assays were performed.

Results

Engase knockout worm's adaptivity to environmental stresses (heat and osmotic) was significantly improved, and its longevity was also increased mi

SUBMITTER: Lu X 

PROVIDER: S-EPMC12187103 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature