Establishing a living biobank of pediatric high-grade glioma and ependymoma suitable for cancer pharmacology.

ABSTRACT:

Background

Brain tumors are the deadliest solid tumors in children and adolescents. Most of these tumors are glial in origin and exhibit strong heterogeneity, hampering the development of effective therapeutic strategies. In the past decades, patient-derived tumor organoids (PDT-O) have emerged as powerful tools for modeling tumoral cell diversity and dynamics, and they could then help define new therapeutic options for pediatric brain tumors.

Methods

Through an integrative approach based on our expertise and a careful review of the literature about glioblastoma 3D primary cultures, we set up a standardized methodological pipeline for the establishment, characterization, and biobanking of PDT-O through direct 3D in vitro culture of the deadliest pediatric glial brain tumors.

SUBMITTER: Deligne C 

PROVIDER: S-EPMC12187455 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature