Glutamine modulates stress granule formation in cancer cells through core RNA-binding proteins.

ABSTRACT: Cytoplasmic stress granules (SGs) induced by various stresses have been linked to cancer and other disorders. Which active energy pathways are required for SG formation remains unclear. We used nutrient deprivation to show that glutamine is the sole amino acid source governing whether cancer cells form SGs. Metabolic profiling revealed the essential functions of glutamine and glucose in SG formation under limiting metabolic conditions. Providing glutamine during metabolic stress restored ATP levels in cancer cells and revived many essential gene expression patterns. MYC, a known regulator of the shift between glucose and glutamine metabolism, showed increased expression as cells moved to glutamine uptake. Inhibition of MYC prevented SG formation even with glutamine present and increased ce

SUBMITTER: Faber GP 

PROVIDER: S-EPMC12188316 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature