Visualizing stress granule dynamics with an RNA guanine quadruplex targeted ruthenium(ii) peptide conjugate.

ABSTRACT: Stress granules (SGs) are membraneless ribonucleoprotein assemblies that form in response to cellular stress. They have been linked to cell survival and cancer progression, though many questions remain regarding their structure, function and therapeutic potential. Live-cell fluorescence imaging is key to advancing understanding of SGs, but there are very few small-molecule probes reported that selectively image these organelles. RNA G-quadruplex (rG4) folding is believed to play a role in initiation of SG formation. Thus, to create a probe for SGs, we conjugated a G4 binding domain peptide from RNA helicase associated with AU-rich element (RHAU) to a luminescent [Ru(bpy)₂(PIC-COOH)]²⁺, Ru-RHAU. Ru-RHAU is designed to target rG4s and thus SGs in live cells. Studies

SUBMITTER: Curley RC 

PROVIDER: S-EPMC12188320 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature