Application of a high-resolution melt assay for monitoring SARS-CoV-2 variants in Burkina Faso and Kenya.

ABSTRACT: The rapid emergence and global dissemination of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) highlighted a need for robust, adaptable surveillance systems. However, financial and infrastructure requirements for whole-genome sequencing mean most surveillance data have come from higher-resource geographies, despite unprecedented investment in sequencing in low- and middle-income countries (LMICs). Consequently, the molecular epidemiology of SARS-CoV-2 in some LMICs is limited, and there is a need for more cost-accessible technologies to help close data gaps for surveillance of SARS-CoV-2 variants. To address this, we have developed two high-resolution melt (HRM) curve assays that target variant-defining mutations in the SARS-CoV-2 genome, which give unique signature profiles

SUBMITTER: Greenland-Bews C 

PROVIDER: S-EPMC12188703 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature