Solid-state nanopore quantification of discrete sequence motifs from DNA and RNA targets in human plasma.

ABSTRACT: Fast and sensitive detection of target nucleic acid biomarker sequences in complex biofluids is essential for translational diagnostics. In this work, we report on the use of a solid-state nanopore assay to quantitate sequence motifs in human plasma. Extracted DNA or RNA is annealed to a biotinylated DNA oligonucleotide probe and then subjected to single-strand-specific enzymatic digestion to decompose off-target regions. The remaining duplex product is then bound to a protein tag that enables selective detection via resistive pulse sensing. We first demonstrate our approach on single-strand DNA and single-strand RNA spiked into human plasma and then extend the methodology to double-strand DNA, expanding the range of motifs that can be targeted. These advancements position our assay

SUBMITTER: Elaguech MA 

PROVIDER: S-EPMC12189179 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature