Protective Effects of Extracts from Green Leaves and Rhizomes of <i>Posidonia oceanica</i> (L.) Delile on an In Vitro Model of the Human Blood-Brain Barrier.

ABSTRACT: Posidonia oceanica (L.) Delile, a Mediterranean seagrass, is rich in bioactive compounds with anti-inflammatory potential. While marine-derived molecules are increasingly studied, their direct effects on blood-brain barrier (BBB) integrity under inflammatory conditions remain largely unexplored. This study evaluated the ability of aqueous extracts from its green leaves (GLEs) and rhizomes (REs) to protect the BBB using a human in vitro model consisting of brain-like endothelial cells co-cultured with brain pericytes. The model was exposed to TNFα, with or without GLEs or REs. We assessed NO production, endothelial permeability, expression of IL-6, NLRP3, ICAM-1, VCAM-1, CLAUDIN-5, and VE-CADHERIN, and the localization of junctional proteins. TNFα increased NO and IL-6 release, upreg

SUBMITTER: Abruscato G 

PROVIDER: S-EPMC12189290 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature