Unlocking Immune Signatures: Surrogate Markers for Assessing VHSV Vaccine Efficacy in Olive Flounder (<i>Paralichthys olivaceus</i>).

ABSTRACT: Viral hemorrhagic septicemia virus (VHSV) is a major pathogen in Paralichthys olivaceus (olive flounder) aquaculture, necessitating effective vaccines and evaluation methods. This study assessed immune responses following intraperitoneal administration of an inactivated VHSV vaccine and identified surrogate markers of vaccine efficacy. Fish were sampled weekly over 8 weeks. Antigen-specific antibody titers were measured by ELISA, and the expression of seven immune-related genes (CD4, CD8, CD28, IgM, Mx, IFN, and IL-1β) was analyzed in the spleen, kidney, liver, and gill by qPCR. The vaccinated group showed 84% relative survival, while unvaccinated fish experienced 100% mortality. Antibody titers peaked at 4 weeks post-vaccinati

SUBMITTER: Jeong JM 

PROVIDER: S-EPMC12189318 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature