Comprehensive Ubiquitome Analysis of <i>Nicotiana benthamiana</i> Leaves Infected with Tomato Brown Rugose Fruit Virus.

ABSTRACT: Tomato brown rugose fruit virus (ToBRFV) is an important emerging virus that poses a serious threat to the global agricultural economy. Ubiquitination is one of the key post-translational protein modification types in plant responses to biotic stress, but the extent to which ToBRFV infection alters the overall ubiquitination status has not been reported. This study conducted integrated ubiquitome and proteome analyses of Nicotiana benthamiana leaves infected with ToBRFV and identified differentially ubiquitinated proteins. A total of 346 lysine sites on 302 identified proteins were found to be affected, with 260 sites exhibiting upregulated ubiquitination levels in 224 proteins and 86 sites showing downregulated ubiquitination levels in 80 proteins. The differentially ubiquitinated

SUBMITTER: Yang J 

PROVIDER: S-EPMC12189520 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature