Red Sea Bream Iridovirus Stability in Freeze-Thaw Cycles: Quantitative Assays of Infectious Particles.

ABSTRACT: Red sea bream iridovirus is a serious threat to farmed fish, but little is known about how repeated freezing and thawing affect its stability. This study investigated the effects of repeated freeze-thaw cycles on RSIV infectivity by comparing quantitative polymerase chain reaction (qPCR), viability qPCR (vqPCR), and 50% tissue culture infectious dose (TCID₅₀) assays. While qPCR detected high amounts of viral DNA after multiple cycles, both viability qPCR and TCID₅₀ revealed a significant loss of infectivity unless serum was present. Correlation analysis showed a high degree of agreement between vqPCR and TCID₅₀, indicating their high compatibility for assessing viral infectivity. However, the correlations between qPCR and vqPCR, as well as between qPCR and

SUBMITTER: Jeong JM 

PROVIDER: S-EPMC12189574 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature