The Biological Consequences of the Knockout of Genes Involved in the Synthesis and Metabolism of H₂S in <i>Drosophila melanogaster</i>.

ABSTRACT: Here, we describe the effects of double knockout (KO) of the cbs and cse genes, which are responsible for H₂S synthesis through the transsulfuration pathway, and KO of the sulfurtransferase gene (dtst1) in Drosophila melanogaster females. The analysis of H₂S production in flies showed minimal levels in the double- and triple-knockout strains. The double- (cbs-/-; cse-/-) and triple- (cbs-/-; cse-/-; dtst-/-) KO flies exhibited a shortened lifespan and reduced fecundity, and showed dramatic changes in Malpighian tubule morphology. The transcriptomic analysis revealed a profound increase in the expression levels of several genes involved in excretory system function in the double-KO and especially the triple

SUBMITTER: Shilova VY 

PROVIDER: S-EPMC12189691 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature