Description of Two Resistance-Nodulation-Cell Division Efflux Systems Involved in Acquired Antibiotic Resistance: AxySUV in <i>Achromobacter xylosoxidans</i> and AinCDJ in <i>Achromobacter insuavis</i>.

ABSTRACT:

Background/objectives

Achromobacter xylosoxidans and Achromobacter insuavis are emerging opportunistic pathogens. Several Resistance-Nodulation-cell Division (RND) efflux systems are involved in intrinsic or acquired antibiotic resistance (AxyABM, AxyXY-OprZ, and AxyEF-OprN). The aim of this study was to explore the resistance mechanisms in one-step mutants in which the efflux systems described to date are not involved: one mutant of A. insuavis AXX-A (AXX-A-Do1) and two mutants of A. xylosoxidans CIP102236 (CIP102236-El9 and CIP102236-Eo4) selected on fluoroquinolones.

Methods

In vitro mutants were compared to parental isolates by WGS. RT-qPCR and gene inactivation were used to explore the role of the new efflux systems detected.

Results

I

SUBMITTER: Magallon A 

PROVIDER: S-EPMC12189693 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature