Bright-Field Multiplex Immunohistochemistry in Swine PCV2 and PRRSV Lymphadenopathies.

ABSTRACT: Multiplex immunostaining (mIHC) allows the simultaneous detection of multiple antigenic targets within the same tissue section, providing a deeper understanding of spatial variation in cellular distribution. The aim of the present study is to apply this technique to examine the spatial variation of lymphocyte populations in swine lymph nodes during PCV2-SD and PRRSV lymphadenopathy compared with reactive lymphoid hyperplasia. A triple immunohistochemical stain with CD3, CD20 and IBA1 antibodies for the concurrent detection of T lymphocytes, B lymphocytes and macrophages, respectively, was performed. Multiplex immunohistochemistry (mIHC) revealed that, compared to reactive hyperplasia, the most significant changes in lymph node cell populations occurred in the follicles for both PCV2 and PR

SUBMITTER: D'Annunzio G 

PROVIDER: S-EPMC12189695 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature