Large-Scale Expansion of Suspension Cells in an Automated Hollow-Fiber Perfusion Bioreactor.

ABSTRACT: Bioreactors enable scalable cell cultivation by providing controlled environments for temperature, oxygen, and nutrient regulation, maintaining viability and enhancing expansion efficiency. Automated systems improve reproducibility and minimize contamination risks, making them ideal for high-density cultures. While fed-batch bioreactors dominate biologics production, continuous systems like perfusion cultures offer superior resource efficiency and productivity. The Quantum hollow-fiber perfusion bioreactor supports cell expansion via semi-permeable capillary membranes and a closed modular design, allowing continuous media exchange while retaining key molecules. We developed a multiple-harvest protocol for suspension cells in the Quantum system, yielding 2.5 × 10¹⁰ MEL-745A cells

SUBMITTER: Brauchle E 

PROVIDER: S-EPMC12189701 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature