The Genetic Background and Culture Medium Only Marginally Affect the In Vitro Evolution of <i>Pseudomonas aeruginosa</i> Toward Colistin Resistance.

ABSTRACT: Background/Objectives: Colistin is a last-resort treatment for Pseudomonas aeruginosa multidrug-resistant infections, but resistance to it is emerging. While colistin resistance in P. aeruginosa is typically associated with chromosomal mutations inducing lipopolysaccharide (LPS) aminoarabinosylation, other mutations unrelated to LPS modifications have been proposed to influence the extent of colistin resistance. Here, we examined whether the genetic background and culture conditions affect the evolution of high-level colistin resistance in this bacterium. Methods: We performed in vitro evolution experiments in the presence or absence of increasing colistin concentrations with two phylogenetically distant reference strains in a standard laboratory medium and in t

SUBMITTER: Cervoni M 

PROVIDER: S-EPMC12189927 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature