Genomic Characterization and Pathogenicity of a Novel Birnavirus Strain Isolated from Mandarin Fish (<i>Siniperca chuatsi</i>).

ABSTRACT:

Background

Birnaviruses infect a wide range of aquatic and terrestrial hosts, including several economically important fish species. This study aimed to isolate and characterize a novel birnavirus strain from mandarin fish (Siniperca chuatsi), a high-value freshwater species in Chinese aquaculture.

Methods

A novel strain, designated mandarin fish birnavirus (MFBV), was isolated from diseased fish and propagated in SCK cells. The complete genome was determined using high-throughput sequencing and RACE. Viral replication kinetics, tissue distribution, and pathogenicity were assessed through in vitro infection, RT-qPCR, histopathology, and experimental challenges. In addition, disinfectant sensitivity and environmental stability were evaluated.

Results

The MFBV ge

SUBMITTER: Zhang H 

PROVIDER: S-EPMC12191561 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature